Glucose dehydrogenase
2025-08-06
2×PCR Super MasterMix
2025-08-07
Glucose dehydrogenase
2025-08-06
2×PCR Super MasterMix
2025-08-07

Manual

Product Number: NG0105

Shipping and Storage

-20 ℃, dry ice transportation.

Component

ComponentVolume
Tn5 transposase50μL
5×FA Reaction Buffer1.0mL
10×TPS buffer solution1.0mL

Description

Tn5 transposase is a highly active mutant of wild-type Tn5 transposase, which can efficiently insert Tn5 transposons into target sequences. Tn5 transposase recognizes the inner end (IE), outer end (OE), and chimeric end (ME) sequences of Tn5 transposase sequences, with the highest in vitro transposition efficiency achieved by fragments containing ME sequences. The insertion site of Tn5 transposase has high randomness, making it widely used in fields such as in vitro transgenic (integration of exogenous genes into host cells) and second-generation sequencing library construction.

Application

In vitro genetic modification (integration of exogenous genes into host cells), high-throughput sequencing, CUT&Tag.


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