NGS DNA Library Prep End Repair Kit for llumina
2025-07-21
Product Number: NG0105
Shipping and Storage
Store at -20℃ and transport on dry ice.
Components
| Component | 100T |
| Tn5 transposase | 50μL |
| 5×FA Reaction Buffer | 1.0mL |
| 10 x TPS buffer solution | 1.0mL |
Description
Tn5 transposase is a highly active mutant of wild-type Tn5 transposase, which can efficiently insert Tn5 transposons into target sequences. Tn5 transposase recognizes the inner end (IE), outer end (OE), and chimeric end (ME) sequences of Tn5 transposase sequences, with the highest in vitro transposition efficiency achieved by fragments containing ME sequences. The insertion site of Tn5 transposase has high randomness, making it widely used in fields such as in vitro transgenic (integration of exogenous genes into host cells) and second-generation sequencing library construction.
Application
In vitro genetic modification (integration of exogenous genes into host cells), high-throughput sequencing, CUT&Tag.
Product source
Escherichia coli strain containing Tn5 transposase sequence.
Stock solution
50mM Tris-HCl (pH 8.0 @ 25°C), 100mM NaCl, 0.1mM EDTA, 0.1% Triton X-100, and 50% glycerol (V/V).
Unit Definition
One unit of Tn5 transposase refers to the amount of enzyme required to completely cleave 1 μ g of DNA fragments containing recognition sequences under 37°C conditions for one hour.
