Product Number: PCK244
Shipping and Storage
Please send in dry ice and immediately store all components in a -20℃ constant temperature refrigerator after receiving the reagent kit, which can be stored for 6 months. If you need to store it for a longer period of time, please store it below -70℃.
Components
Component | PCK244 24rxns | PCK244 96rxns |
Cell Lysis Buffer | 240μl | 960μl |
Cell Lysis Enzyme | 16μl | 64μl |
Pre-Amp Buffer | 120μl | 480μl |
Pre-Amp Enzyme Mix | 7μl | 28μl |
Amplification Buffer | 1.5ml | 4×1.5ml |
Amp Enzyme Mix | 50μl | 200μl |
Description
The single-cell whole genome amplification kit can achieve whole genome amplification using single cells or trace samples as templates. The single-cell amplification reaction time is short, with a total process of about 3 hours. After lysis, pre amplification, and amplification, 2-5μg of genomic DNA can be obtained, with a size of around 200-1500bp. The amplified products can be widely used for second-generation sequencing, large segment copy number variation analysis, SNP typing, qPCR analysis, gene chip analysis, etc.
Self provided instruments and reagents
Note
This product has extremely high detection sensitivity, and experimental operations should be completed in a positive pressure ultra clean workbench or clean environment. The concentration of amplification reaction products is high, and isolation should be done to avoid aerosol pollution caused by amplification products.
Operation process diagram