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Single Cell WGA Kit

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PCK244, Single Cell WGA Kit

Product Number: PCK244

Shipping and Storage

Please send in dry ice and immediately store all components in a -20℃ constant temperature refrigerator after receiving the reagent kit, which can be stored for 6 months. If you need to store it for a longer period of time, please store it below -70℃.

Components

ComponentPCK244 24rxnsPCK244 96rxns
Cell Lysis Buffer240μl960μl
Cell Lysis Enzyme16μl64μl
Pre-Amp Buffer120μl480μl
Pre-Amp Enzyme Mix7μl28μl
Amplification Buffer1.5ml4×1.5ml
Amp Enzyme Mix50μl200μl

Description

The single-cell whole genome amplification kit can achieve whole genome amplification using single cells or trace samples as templates. The single-cell amplification reaction time is short, with a total process of about 3 hours. After lysis, pre amplification, and amplification, 2-5μg of genomic DNA can be obtained, with a size of around 200-1500bp. The amplified products can be widely used for second-generation sequencing, large segment copy number variation analysis, SNP typing, qPCR analysis, gene chip analysis, etc.

Self provided instruments and reagents

  1. PCR instrument
  2. Reaction tube: It is recommended to use a low adsorption tube
  3. Gun head: It is recommended to use high-quality filtering gun head
  4. Micro centrifuge, vortex mixer

Note

This product has extremely high detection sensitivity, and experimental operations should be completed in a positive pressure ultra clean workbench or clean environment. The concentration of amplification reaction products is high, and isolation should be done to avoid aerosol pollution caused by amplification products.

Operation process diagram

PCK244, Single Cell WGA Kit


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