Manual

Storage Condition

-20°C

Components

• Phi29 DNA Polymerase, 10 U/μl
• 10x Phi29 Reaction Buffer
• BSA, 10 mg/ml

Description

Phi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29 and expressed by E. coli using gene recombination technology. This product has the ability of efficient continuous DNA synthesis and chain replacement, and has the function of 3 '→ 5' exonuclease reading, with high fidelity. This product can be applied to replication reactions requiring strong replacement and continuous synthesis and high fidelity replication under medium temperature conditions, such as plasmid replication, whole genome amplification, etc.

Unit Definition

At 30°C, within 10 minutes, the amount of enzyme required to add 0.5 pmol of deoxynucleotides to the acid insoluble precipitate is defined as 1 active unit (U).

Thermal inactivation

Incubation at 65°C for 10 minutes can inactivate.

Quality Control

After multiple column purification, the purity of SDS-PAGE was more than 95%; No endonuclease activity and no host residual DNA were detected.

The enzyme buffer contains reductant DTT to ensure its maximum enzyme activity. If the buffer is not fresh or after repeated freezing and thawing, 4 mm DTT should be added before use.

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