Super Fi Amplification Mix for NGS
2025-01-14
Animal Detection Probe Mixture (UNG)
2025-01-14Product Number: PCK48
Shipping and Storage
Box 1 -20℃ storage, dry ice transportation.
Box 2 2-8℃, transported in ice bags
Components
- Box 1
| Component | PCK48 24rxns | PCK48 96rxns |
| FER Buffer | 240 μL | 960 μL |
| FER Enzyme Mix | 120 μL | 480 μL |
| T4 DNA Ligase | 72 μL | 288 μL |
| T4 DNA Ligase Buffer | 336 μL | 672 μL×2 |
| 2×Super HiFi PCR Mix | 600 μL | 1.2 mL×2 |
| Neutralization Reagent | 120 μL | 480 μL |
- Box 2
| Component | PCK48 24rxns | PCK48 96rxns |
| Magnetic Bead | 1.5 mL×2 | 4 mL×3 |
Description
The Universal DNA Library Kit (Illumina&MGI) is a second-generation sequencing enzyme digestion library kit developed for Illumina and MGI sequencing platforms. It includes three modules: DNA fragmentation/end repair/addition of A, adapter connection, and pre mixed enzymes required for library enrichment in library construction.By controlling the reaction time, sample DNA fragments from different sources such as 0.1ng-1μg genomic DNA, PCR amplification products, and FFPE can be reduced to small fragments, avoiding tedious ultrasound processes and instrument dependence.This kit simplifies the purification steps and shortens the library construction time. In the amplification module, high fidelity DNA polymerase is used for library enrichment, and PCR amplification is performed without preference, ensuring the accuracy of sequencing results.
Features
- Accurate enzyme digestion, fragment end repair and A tube completion, without the need for purification and direct connection to the connector.
- After connecting the joint, use matching magnetic beads for direct sorting (optional).
- High fidelity enzymes for PCR enrichment and amplification minimize amplification preference.
- Suitable for building libraries for different species such as humans, animals, plants, microorganisms, etc. The resulting library is applicable to various platforms such as Illumina and MGI.
Schematic diagram of DNA database construction process
DNA library construction process
Please read this operating instruction carefully before the experiment and choose the operating plan based on the type of sequencing platform used. Before the experiment starts, clarify the nucleic acid concentration, and the input amount of this reagent kit is 0.1ng-1μg. The recommended sample size is 1ng-500ng DNA. DNA solution does not contain chelating agents, if DNA dissolves in 1×TE or EDTA containing solutions, it is recommended to use magnetic beads for purification or add Neutralization Reagent.
