Manual

Product Number: PCK246

Storage

Store at -20 ℃ and transport on dry ice.

Components

Note: This kit is suitable for constructing human genomic DNA libraries, with a starting template DNA input of 5ng. Our company also has NGS TPH DNA Library Preps Kit for lllumina (1ng) for 1 ng (PCK247). To obtain a high-quality library, it is recommended to use different reagent kits for different DNA starting quantities.

Description

This kit is a specialized kit developed specifically for the Illumina high-throughput sequencing platform, providing the enzyme premix system and reaction buffer required for constructing genomic DNA libraries, including all components except PCR primers. Compared with traditional library construction kits, this kit adopts a novel transposase method for library construction, which can complete DNA fragmentation, end repair, and connector connection reactions through a simple enzymatic reaction, significantly reducing the use of templates, reducing experimental steps, and shortening library construction time; The use of high fidelity DNA polymerase for library enrichment and preference free PCR amplification has expanded the coverage area of the sequence, enabling efficient preparation of DNA libraries for the Illumina second-generation sequencing platform. This reagent kit is suitable for a starting template DNA input of 5ng. All reagents in the reagent kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent possible.

Features

1. DNA fragmentation and splicing are completed in one step.
2. Ultra fidelity amplification minimizes amplification preference to the greatest extent possible.

Schematic diagram of DNA library construction process