2x Hotstar PCR Master Mix, with blue dye
2025-01-03
Fast NGS Library Kit for Illumina MGI
2025-01-13Product Number: PCK85
Storage
Store at -20 ℃ and transport on dry ice.
Components
| Component | PCK85 | PCK85 |
| End Prep Enzyme Mix | 48 μl | 192 μl |
| 10×End Repair Reaction Buffer | 200 μl | 800 μl |
| T4 DNA Ligase | 48 μl | 192 μl |
| T4 DNA Ligase Buffer | 400 μl | 2×800 μl |
| 2×HiFidelity PCR Mix | 600 μl | 2×1.2 ml |
Description
This kit provides the enzyme premix system and reaction buffer required for DNA library construction, including all components except connectors and PCR primers, for the construction of Illumina second-generation sequencing platform DNA library. Compared with general library building methods, this kit is simple and convenient to operate, greatly shortening the library construction time. In addition, the kit uses high-fidelity DNA polymerase for library enrichment, with no preference for PCR amplification, expanding the coverage area of the sequence, and can efficiently prepare a DNA library for the Illumina second-generation sequencing platform. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability of library construction to the greatest extent possible.
Features
- End leveling, phosphorylation, and addition of A are completed in one step.
- No need for purification, add connectors directly.
- Ultra fidelity amplification minimizes amplification preference.
- Supports multiple samples, and the resulting library can be used for sequencing platforms such as Illumina GAIIx, HiSacnSQ, HiSeq 2500/2000/1000, and MiSeq sequencing.
Schematic diagram of DNA database construction process
