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Proper selection of reverse transcription is a critical step in the reverse transcription reaction. Reverse transcription is an enzyme class capable of transcribing RNA into DNA. When selecting a reverse transcription, one needs to consider its specificity, activity and stability.
Primers refer to large molecules with specific nucleotide sequences that are stimulated and synthesized at the beginning of nucleotide polymerization, and are connected to reactants in the form of hydrogen bonds. Such molecules are called primers.
Nucleic acid electrophoresis is a commonly used molecular biology technique for separating and analyzing DNA and RNA molecules. It is based on the principle that nucleic acid molecules migrate at different speeds in an electric field, and the nucleic acid samples are separated into different band profiles by electrophoresis instruments, thus realizing qualitative and quantitative analysis of nucleic acid molecules. This article will introduce the principle of nucleic acid electrophoresis and common electrophoresis methods.
With the outbreak of the COVID-19, scientists around the world have rapidly embarked on vaccine development. Among them, mRNA vaccines have become an emerging vaccine technology that has attracted much attention. In this paper, we will introduce the development process and challenges of mRNA vaccines.
Ribonuclease inhibitor (RI) is a large (~450 residues, ~49 kDa), acidic (pI ~4.7), leucine-rich repeat protein that forms extremely tight complexes with certain ribonucleases.