Yellow Fever PCR Test Kit is a diagnostic kit used for detecting Yellow Fever Virus (YFV). It mainly employs Real-Time Polymerase Chain Reaction (Real-Time PCR) technology to qualitatively detect the specific RNA of Yellow Fever Virus. Here are some key points about Yellow Fever PCR Test Kits:

1. Purpose

• Clinical Diagnosis: It is used to qualitatively detect Yellow Fever Virus RNA from clinical samples such as whole blood, serum, plasma, and urine. It is suitable for individuals who meet the clinical and/or epidemiological criteria for Yellow Fever.
• Research Use: Some kits are for research use only and not for clinical diagnosis.

2. Detection Principle

• Real-Time PCR Technology: By using fluorescently labeled primers and probes, the kit monitors the changes in fluorescent signals during the PCR amplification process, thus achieving qualitative detection of Yellow Fever Virus RNA.

3. Product Features

• Ready-to-Use: Users only need to provide the RNA template of the sample. The operation is simple, and the quantification is accurate and fast.
• High Specificity: The primers and probes are optimized to be highly specific and will not cross-react with the RNA of other organisms.
• High Sensitivity: The sensitivity can reach several hundred copies per reaction. Some kits can even reach 100 copies per reaction.
• Internal and Positive Controls: The kit usually includes internal and positive controls to ensure the reliability of the detection process and the accuracy of the results.

4. Kit Components

• Main Components: Include PCR buffer, dNTP mixture, primers, probes, Taq DNA polymerase, Mg2+, etc.
• Other Components: Internal control, positive control, PCR-grade water, etc.

5. Sample Types

• Applicable Samples: Whole blood, serum, plasma, urine, etc.

6. Compatibility with Detection Platforms

• Multiple Real-Time PCR Platforms: Compatible with various mainstream real-time PCR instruments, such as Mx 3005P™ QPCR System, VERSANT® kPCR Molecular System AD, ABI Prism® 7500 SDS, etc.

7. Storage and Transportation

• Storage Conditions: Generally need to be stored at -20℃ in the dark to avoid repeated freezing and thawing.
• Transportation Conditions: Transported at low temperature and in the dark.

8. Shelf Life

• General Shelf Life: Under appropriate storage conditions, the shelf life of the PCR kit is usually 1 year.

9. Clinical Performance

• Clinical Sensitivity and Specificity: The clinical sensitivity of RealStar Yellow Fever Virus RT-PCR Kit 1.0 is 100% (95% CI 73.5-100%), and the clinical specificity is 100% (95% CI 92.1-100%).

10. Price

• Different Specifications: The price varies according to different packaging specifications. For example, the price of the 96-test kit of RealStar Yellow Fever Virus RT-PCR Kit 1.0 is 1,440.00 euros.

11. Scope of Application

• Global Yellow Fever Laboratory Network: Some kits have been evaluated by top laboratories in the Global Yellow Fever Laboratory Network and meet the standards of the World Health Organization (WHO).

12. Other Information

• Recommended by UNICEF: Some kits are recommended by UNICEF for the monitoring and diagnosis of Yellow Fever.

This information can help you better understand the purpose, features, and usage of Yellow Fever PCR Test Kits. If you have more specific questions, it is recommended to consult the relevant suppliers or professional laboratories.