T7 High Yield RNA Transcription kit
2021-12-24
Benzo Nuclease
2021-12-24
Product Number: TR01
Shipping and Storage
-20℃.
Components
| Components | TR01 |
| T7 RNA Polymerase（50U/μl） | 100μl |
| 5×Transcription Buffer | 1ml |
Description
T7 RNA polymerase is a DNA dependent 5'→ 3'rna polymerase that highly specifically recognizes the T7 promoter sequence. T7 RNA polymerase can catalyze the incorporation of NTP downstream of T7 promoter of single stranded or double stranded DNA to synthesize RNA complementary to template DNA downstream of T7 promoter.
Source
It is expressed by Escherichia coli and the expressed gene is bacteriophage T7 RNA polymerase gene.
Concentration
50U/μl
Purity
No DNA endonuclease and exonuclease, no RNase.
Feature
T7 RNA polymerase can recognize modified NTPs, such as biotin labeled, digoxigenin labeled, fluorescein labeled NTPs, and can be used for the synthesis of various labeled RNAs. At the same time, it has high specificity for T7 promoter.
Application
For RNA synthesis, the synthesized RNA can be used or used as: hybridization probe, genomic DNA sequence analysis, RNase protection assay, antisense RNA synthesis, as RNA template for in vitro translation, substrate for RNA splicing research, RNA secondary structure and RNA protein interaction, nucleic acid amplification analysis, siRNA, miRNA and other small RNAs.
Unit definition
The amount of enzyme required to catalyze the incorporation of 1 nmol of AMP into polynucleotides within 60 min at 37 ° C was defined as 1 active unit.
Inactivation or inhibition
Heating at 70℃ for 10 min can inactivate T7 RNA polymerase. Addition of an appropriate amount of EDTA can also inactivate T7 RNA polymerase. Chelating agents, sodium, potassium or ammonium salts with concentrations greater than 150mm can significantly inhibit the activity of T7 RNA polymerase.
Order
| TR01-01 | T7 RNA Polymerase(50U/μl) | 100ul |
| TR01-02 | T7 RNA Polymerase(50U/μl) | 1ml |
| TR01-03 | T7 RNA Polymerase(50U/μl) | 10ml |
| TR01-04 | T7 RNA Polymerase(50U/μl) | 50ml |
| TR01B | 5xTranscription Buffer | optional |
