Magbead Plant DNA Kit (96 Auto Plate)
2025-10-24Low dsRNA High Yield T7 RNA Co-Transcription Kit, with CAP 1 GAG
Product Number: CA341
Shipping and Storage
Store at -20℃±5℃.
Component
| Component | CA341-20T |
| Enzyme Mix 2.0 | 20μL |
| 10×Transcription Buffer-A | 40μL |
| 10×Transcription Buffer-B | 40μL |
| ATP (200mM Tris Solution) | 20μL |
| UTP (200mM Tris Solution) | 20μL |
| GTP (200mM Tris Solution) | 20μL |
| CTP (200mM Tris Solution) | 20μL |
| N1-Me-Pseudo UTP (200mM Tris Solution) | 20μL |
| CAP1 GAG (100mM) | 20μL |
| Control template (100ng/μL） | 20μL |
| Lithium Chloride Precipitation Solution | 1mL |
| DNase I, RNase-free (2U/μL) | 40μL |
| RNase Free Water | 1mL |
Description
As a biomacromolecule, mRNA can be synthesized on a large scale through in vitro transcription (IVT). The T7 promoter is currently one of the most efficient promoters for transcription. Therefore, using T7 RNA polymerase (T7 RNA polymerase) for in vitro transcription can easily and quickly obtain a large number of RNA molecules. This kit optimizes the co transcription reaction system and uses T7 RNA polymerase 2.0, a T7 RNA polymerase mutated enzyme obtained through molecular evolution, which can significantly reduce the production of dsRNA during transcription.
This co transcription kit can use DNA containing T7 promoter sequence, AG promoter sequence, and poly (A) tail coding region as templates, and incorporate CAP1 GAG cap analogs during transcription to obtain mRNA with 5 ‘- Cap1 and 3’ – poly (A) tails. The 5 ‘- Cap1 structure can prevent mRNA from being degraded by nucleases, thereby maintaining mRNA stability and initiating translation, which has a significant impact on mRNA stability, translation efficiency, and immunogenicity in vivo or in cells.
This reagent kit can transcribe 150-250μg of RNA in one reaction, and the co transcription cap rate is greater than 95%. The synthesized RNA can be used for research on RNA structure and function, RNA enzyme protection, probe hybridization, etc RNAi、 Downstream applications such as microinjection and in vitro translation.
Application
Synthesize single stranded RNA with CAP1 GAG structure, which can be replaced with other cap analogs for use.
