Manual

Product Number: DI05

Shipping and Storage

 -20℃，Valid for 12 months.

Components

Description

DNase I is a non-specific nuclease cleavage enzyme, mostly derived from recombinant E. coli strains, containing MBP fusion clones of bovine pancreatic DNase I. DNase I can be used to degrade single or double stranded DNA, based on the principle that DNase I hydrolyzes phosphate diester bonds to produce single or oligonucleotides with 5 '- phosphate groups and 3' - OH.

Both Mg²⁺ and Mn²⁺ can activate the activity of DNase I, and the concentration of Ca²⁺ directly affects the activity of the enzyme. When Mg²⁺ is present, it can randomly generate incisions on each single strand of double stranded DNA; In the presence of Mn²⁺, double stranded DNA can be broken, resulting in DNA fragmentation.

DNase I solution (1mg/ml) is composed of DNase I, enzyme protection solution, preservatives, etc., with a concentration of 1mg/ml, used for single stranded DNA, double stranded DNA, chromatin, RNA: DNA hybrid strands. It is commonly used for the preparation of DNA free RNA, reverse transcription, and in vitro transcription experiments.

Unit definition

1 unit refers to the amount of enzyme required to completely degrade 1μg of pBR322 DNA in a 50μl reaction system at 37℃ for 10 minutes.