animal origin free

Description

BspQI is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli.

Recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly(A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends.

This product is produced in accordance with GMP process requirements and supplied in a liquid format.

Source

Recombinant E. coli with BspQI gene.

Reaction Temperature

50℃.

Storage Buffer

20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol.

Reaction Buffer:

10× Digestion Buffer 500mM Tris-HCl, 1M NaCl,100mM MgCl2, 1mg/mL OsrHSA, pH7.9@25℃.

Unit Definition

1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system.

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

Applications

1. Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);

2. Digestion of DNA to obtain specific sticky ends.

Protocol

50 μL reaction system.

This step is suitable for linearization of 1 μg DNA (≥100 nt) and can be scaled up according to experimental needs.

1）Add the following components in sequence:

Components	Volume
Plasmid DNA	1-2 μg
10×Digestion Buffer	5.0 μL
BspQI (10 U/μL)	1.0 μL
RNase-free ddH2O	Up to 50 μL

2）Incubate at 50°C 1h.

3）DNA linearization is complete, and subsequent experiments can be performed.

Order

M062011

BspQI

BspQI (10U/μl), 10xBspQI Reaction Buffer