Hot Exo- DNA Polymerase
2025-02-28
BspQI, 20ul
2025-03-07
Product Number: M062011
Shipping and Storage
Ice pack transportation, stored at -20℃, with a shelf life of 1 year.
Component
| Component | 5KU | 250 KU | 1 MU |
| BspQI (200 U/μL) | 25μL | 1.25mL | 5mL |
Description
This product is a restriction endonuclease type II, derived from a recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed in E. coli. Its recognition sequence is 5 '- GCCTTCN1/N4-3', mainly used for enzyme digestion of plasmids to prepare poly (A/T/G/C) terminated linearized DNA fragments and obtain specific sticky ends.
Specification
| Source | E. coli |
| Reaction Temperature | 50℃ |
| Storage Conditions | 20mM Tris-HCl, 0.5 M KCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol |
| Unit Definition | One unit of activity is defined as the amount of enzyme required to digest 1μg of λ DNA within 1 hour at 50℃ in a 50μL system |
Note: For specific product quality inspection data and more indicators, please refer to the batch quality inspection report.
Unit definition
1 unit: The amount of enzyme required to digest 1μg of λDNA within 1h at 50℃ in a 50μL system.
