Manual

Product Number: DB003

Shipping and Storage

-20℃.

Components

Description

This enzyme is obtained by expressing the phosphatase gene isolated from the Antarctic cold loving strain TAB5 in Escherichia coli and purifying it multiple times. Phosphatases can catalyze the removal of 5 ‘phosphate groups at the ends of DNA or RNA. After treating the vector DNA with thermosensitive phosphatase during cloning, its fragment lacks the necessary 5 ‘phosphate terminus for ligase, so the vector cannot perform self ligation, which can greatly reduce the background of vector self ligation. This enzyme can act on the 5 ‘protruding end, concave end, and flat end.

Application

1. Remove the phosphate group at the 5 ‘end of the carrier to reduce the background of self connection;
2. Remove residual dNTP and pyrophosphate from PCR products;
3. Remove the phosphate group at the 5 ‘end of the DNA probe and prepare the optimal substrate for phosphorylation labeling at the 5’ end;
4. Protein dephosphorylation.