Manual

Product Number: CK03092

Characteristics:

Source: Chinese Hamster Ovary cell line
Description: Mouse LIF (Pro25-Phe203), with a C-terminal 6-His tag.
Accession #: P09056
Predicted molecular mass: 20.6 kDa

Specification:

Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured in the M1 cell differentiation assay. The ED50 is ≤0.2 ng/ml.

Formulation: Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH 7.4

Size: 50 μg/vial, 1 mg/vial

Handling and Storage:

Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability:

• 36 months at 2°C to 8°C in lyophilized state
• 6 months at -20°C to -80°C under sterile conditions after reconstitution
• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.