Manual

Product Number: CK04503

Characteristics:

Source: Chinese Hamster Ovary cell line
Description: Human Transferrin (Val20-Pro698) with a C-terminal linker GGGGGS and Human IgG1 (Asp104-Lys330)
Accession # P02787
Predicted molecular mass: 101.1 kDa (monomer)

Specification:
Appearance: White powder, clear colorless liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤1 EU/mg by the LAL method
Activity: Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is 0.5-2 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
Formulation: Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size: 100 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability:

• 36 months at 2°C to 8°C in lyophilized state
• 6 months at -20°C to -80°C under sterile conditions after reconstitution
• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.