Manual

Product Number: CK03501

Characteristics:

Source: Chinese Hamster Ovary cell line
Description: Human R-Spondin1 (Ser21-Ala263)
Accession #: Q2MKA7
Predicted molecular mass: 26.7 kDa

Specification:

Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured by its ability to induce Topflash reporter activity in CHO cells in the presence of 50 ng/ml wnt3a. The expected ED50 is ＜1.0 μg/ml.

Formulation: Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4

Size: 50 μg/vial, 1 mg/vial

Handling and Storage:

Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability:

• 36 months at -20°C to -80°C in lyophilized state
• 6 months at -20°C to -80°C under sterile conditions after reconstitution
• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.