Manual

Product Number: CK00421H

Characteristics:

Source: E. coli
Description: Human BMP2 (Gln283-Arg396)
Accession # P12643
Predicted molecular mass: 12.9 kDa (monomer)

Specification:

Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤100 EU/mg by the LAL method
Activity: Measured alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 is ≤0.5 μg/ml
Formulation: Lyophilized from a 0.2 μm filtered solution containing 0.1M Acetic acid, 5% Trehalose, pH 3.0
Size: 10 μg/vial, 50 μg/vial, 1 mg/vial

Handling and Storage:

Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to 8°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.