Manual

Product Number: CK07512

Characteristics:

Source: Chinese Hamster Ovary cell line
Description: Bovine Fetuin A (I19-I359), with a C-terminal 10-His tag
Accession # P12763
Predicted molecular mass: 37.7 kDa

Specification:
Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥ 95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤0.5 EU/mg by the LAL method
Activity: Measured in a cell proliferation assay using B16-F1 cells. Recombinant Bovine Fetuin A (His Tag) (Cat. No. Y07512) stimulates adhesion of B16-F1 cells. The ED50 for this effect is ≤100 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
Formulation: Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, pH 7.4
Size: 100 mg/vial, 1 g/vial, 10 g/vial, 100 g/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to 8°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.