M050802 Luciferase mRNA

Product Description

Luciferase is a general term for the enzymes that can produce biofluorescence in nature, among which the most representative is the Luciferase from the body of the North American firefly, Luciferase. The firefly can display Luciferase activity without post translation modification

The sequence of this product is from the North American firefly (Photinus pyralis), and point mutation is carried out on the basis of the wild type sequence, which significantly improves the thermal stability and suitable pH value range of the protein. This product is synthesized by using the T7 High Yield RNA Transcription Kit, and Cap 1 Capping System is modified. It is a mature mRNA with 5 'Cap 1 structure and 3' poly (A) tail, and the introduction of Cap1 structure and poly (A) tail structure, It increases the stability and translation efficiency of Luciferase mRNA, making it an ideal tool for studying transfection and protein translation

Product Features

1. The Cap 1 structure is more suitable for mammalian systems than the Cap 0 structure (ARCA and m7Cap), with higher transcription efficiency. The addition of Poly (A) tail inhibits RNA mediated innate immune activation, increasing the stability and lifespan of mRNA in vivo and in vitro. Poly (A) also plays an important role in improving translation initiation efficiency

2. The experimental method is simple and fast, with stable results and good reproducibility

3. High transfection efficiency

Product Application

1. As a reporting material for gene regulation and functional research

2. Suitable for detecting mRNA transmission, translation efficiency, cell viability, etc

Quality Control

No RNA enzyme residue, single mRNA electrophoresis band

Storage Condition

Store at -20 ℃

Packing Size

Note

The translation and expression of mRNA in vivo are directly related to the amount of mRNA added