Manual

Product Number: PCM01G

Storage

-20℃.

Components

Description

This product contains Taq DNA polymerase dNTPs, MgCl₂, Reaction buffer with a concentration of 2 ×. It has the advantages of speed, simplicity, high sensitivity, strong specificity, and good stability, which can minimize human errors to the greatest extent possible.

This product is easy and fast to use, and can avoid contamination during PCR operation. To use, simply take an appropriate amount of 2 × Taq PCR MasterMix solution, add the template and primer, and add ddH₂O to make up the volume, so that the MasterMix solution concentration is 1 × and the reaction can be carried out. Please ensure sufficient dissolution and mixing before use.

This product comes in two types: dye containing and dye free. There are two types of dyes (blue dye and yellow dye) in dye containing products, which are separated during electrophoresis to monitor migration progress. The mobility of blue dye in 1% agarose gel is the same as that of 3-5 kb DNA fragment. Yellow dye migrates faster than primer in 1% agarose gel (<50bp).

Application

1. Genetic testing: The error between different batches of this product is very small, making it particularly suitable for large-scale genetic testing, semi quantitative PCR experiments, and trace DNA detection.
2. This product is suitable for PCR amplification of DNA fragments, DNA labeling, primer extension, sequence determination, etc. The PCR product with band A can be directly cloned using TA after purification.

Quality control

No exogenous nuclease activity detected; PCR method for detecting residual DNA without host; Can effectively amplify single copy genes in the human genome; After being stored at room temperature for one week, there was no significant change in activity.