Manual

Product Number: PCM13B

Shipping and Storage

-20°C. Store at 2-8°C for frequent use.

Components

Note: The 2×Hotstar PCR Master Mix, with blue dye contains Golden Star Taq DNA Polymerase, 3.4mM MgCl₂ and 400µM

each dNTP.

Description

2×Hotstar PCR Master Mix is a premixed system consisting of Golden Star Taq DNA Polymerase, PCR Buffer, Mg²⁺, dNTPs, PCR stabilizers and reinforcers. The premixed PCR mixture makes the operation easier and faster. Minimizes human error and pollution. The Golden Star Taq DNA Polymerase is a chemically modified, new high efficiency Taq DNA Polymerase that completely blocks the enzyme activity at room temperature, making the enzyme inactive at low or normal temperatures. In order to effectively avoid the non-specific amplification caused by the non-specific combination of primer and template or primer dimer at room temperature, the activation of the enzyme must be incubated at 95°C for 10 min. The unique buffer system enables the enzyme to be widely used, enabling efficient amplification of templates with high GC content, complex secondary structure and low copy. The unique Master Mix formula makes the whole reaction system more stable. PCR amplification with this product, PCR product 3' end with an "A" base, can be directly used for T/A cloning. This product does not contain dye, PCR procedure after bunching can be added according to the need of sample loading buffer after electrophoresis operation. This product has strong specificity and can be directly used for downstream cloning or chip hybridization experiments without the need of agarose gel recovery after PCR amplification. It is mainly used for conventional PCR, RT-PCR, multiple PCR and gene chip detection, especially for PCR reaction with high specificity requirements.

Quality Control

No exogenous nuclease activity was detected. No host residual DNA was detected by PCR. It can effectively amplify single copy genes in human genome. 2-8°Cstore three months, no significant activity change.