Manual

Product Number: BS06

Shipping and Storage

Store at -30 ~ -15 ℃ and transport at ≤ 0 ℃.

Components

Note:1) Store in 10 mM Tris HCl pH 7.5, 50 mM KCl, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol.

2)Self provided material: Reagent:dNTP Mix，FIP/BIP Primers，F3/B3 Primers，LoopF/LoopB Primers，Nuclease-free ddH2O。

Instrument: qPCR instrument, PCR instrument or water bath.

Description

Bst 2.0 Pro DNA Polymerase is a directed modification of a large fragment of Bacillus stearothermophilus DNA polymerase, which exhibits 5 '→ 3' DNA polymerase activity and strong strand displacement activity, but lacks 5 '→ 3' exonuclease activity. Bst 2.0 Pro DNA Polymerase can be used for isothermal amplification reactions, such as LAMP (Loop Mediated Isothermic Amplification), HDA (Helicate Dependent Amplification), RCA (Rolling Circle Amplification), etc. Compared with Bst 2.0 DNA Polymerase, Bst 2.0 Pro DNA Polymerase, combined with a new generation of hot start technology, can inhibit polymerase activity at temperatures below 50 ℃ and rapidly release enzyme activity above 50 ℃. It has higher amplification speed, specificity, salt resistance, and thermal stability, and supports the preparation of reaction systems at room temperature.

Source

Derived from Bacillus stearothermophilus.

Application

This product is suitable for various isothermal amplification reactions such as LAMP, HDA, RCA, etc.

Unit definition

The amount of enzyme required to add 10 nmol of dNTP to acid insoluble precipitate within 30 minutes at 65 ℃ is defined as one active unit (U).

Note

1. This product is for scientific research purposes only and shall not be used for clinical medical diagnosis or other unreasonable purposes.
2. This product is not suitable for PCR reactions.
3. The operating temperature of this product does not exceed 70 ℃.