Manual

Product Number: PCM60

Shipping and Storage

Storage at -20 ± 5 ℃.

Components

Description

Universal Super SYBR Master Mix is a specialized premix for real-time fluorescence quantitative qPCR reactions using the dye method (SYBR Green I).The core component FastStar DNA Polymerase is an antibody based hot start DNA polymerase that can be restored by heating at 95℃ for 5 seconds. It has many advantages such as strong specificity and high detection sensitivity, and is paired with an optimal buffer optimized for qPCR. The reaction solution concentration is 2×。The unique qPCR buffer system of this product, combined with hot start enzymes, effectively inhibits the production of non-specific products and significantly improves the amplification efficiency of qPCR. It is very suitable for qPCR reactions with high specificity, sensitivity, and AT, GC models.

This product is also suitable for qPCR rapid reaction programs, small volume reactions, and can achieve rapid and accurate detection and quantification of target genes without affecting specificity and high sensitivity.Good standard curves can be obtained within a wide quantitative range, with accurate, reproducible, and reliable quantification of target genes. The ROX Reference Dye contained in this product is suitable for most qPCR instruments. This product is not easy to generate bubbles when shaking, and the reaction only requires the addition of primers and templates, which is simple and convenient to operate.

Application

This reagent is used for quantitative amplification of DNA samples and can amplify DNA from most species. Sample types can be genomic DNA, cDNA, plasmid DNA λ DNA, etc.

Note

1. Before use, gently mix upside down and centrifuge briefly before use.
2. This product should avoid repeated freeze-thaw as much as possible, and should not be freeze-thawed more than 10 times to avoid a decrease in enzyme activity.
3. Due to its extremely high detection sensitivity, this product is easily contaminated by aerosols in the air. Therefore, when preparing the reaction system, please use a super clean workbench. During the preparation process, please use a sterilization gun head and reaction tube. If conditions permit, it is recommended to use a dedicated pipette gun and a gun head with a filter element in laboratories.
4. Due to the presence of fluorescent dye SYBR Green I in this product, it is important to avoid strong light exposure when preparing the reaction system.