Product Number:RT05F
Shipping and Storage
Stored at -30~-15℃, Dry ice transportation to avoid repeated freezing and thawing.
Components
Component | RT05F 10000U | RT05F 200KU | RT05F 2000KU |
HiFi II M-MLV Reverse Transcriptase (RNase H-) (Glycerol-free) (200U /μL) | 50μL | 1ml | 10ml |
5×SuperRT Buffer | 1ml | 10ml | 100ml |
Description
HiFi II M-MLV Reverse Transcriptase (RNase H-) (Glycerol-free) is the glycerol free version of HiFi II M-MLV Reverse Transcriptase (RNase H-),it is a reverse transcriptase that recombines and expresses the mutated M-MLV gene using Escherichia coli engineering bacteria,this enzyme can catalyze complementary DNA polymerization reactions using RNA or DNA: RNA hybrid chains as templates.The mutated HiFi II M-MLV (H -) reverse transcriptase RNase H activity is missing, reducing RNA degradation in reverse transcription reactions and making it easier to obtain full-length cDNA.HiFi II M-MLV (H -) reverse transcriptase can synthesize the first strand of cDNA at 55℃, providing higher specificity and stability. It can synthesize up to 12kb of cDNA with high cDNA yield.Suitable for the synthesis of first strand cDNA, RT-PCR, RT-qPCR, construction of full-length cDNA libraries, and application of freeze-dried RT-PCR products.This product does not contain freeze-dried shaping ingredients, and can be customized and added according to needs when applied to freeze-dried products.
Unit definition
Using Poly (A) as the template and oligo (dT) as the primer, the enzyme required to catalyze the incorporation of 1nmol of dTTP within 10 minutes at 37 ℃ is defined as one active unit (U).
Quality Control
The electrophoresis bands of the RNA did not change when 200U of this enzyme reacted with 1µg of 16S, 23S rRNA at 37 ℃ for 1 hour.
Notes