Manual

Product Number: PCK49

Shipping and Storage

Stored at -30~-15℃, valid for 1 year. If frequent use is required, it can be packaged and stored at 2-8℃ to avoid repeated freeze-thaw as much as possible

Components

Description

This product is a specialized reagent kit for one-step RT-qPCR using probe methods (TaqMan, Molecular Beacon, etc.).When using this product for RT qPCR reaction, reverse transcription and quantitative PCR are carried out in the same reaction system, and there is no need to add reagents or open the tube cap during the reaction process, which avoids pollution and improves experimental efficiency.This product has high detection sensitivity, strong fluorescence signal, and high signal-to-noise ratio, making it very suitable for the detection of trace amounts of RNA such as RNA viruses. The special buffer system contained in it can maximize the effectiveness of both reverse transcriptase and DNA polymerase, improving reaction efficiency.Using this product can obtain a wider linear range, more accurate quantification of the target gene, good repeatability, and high reliability. This kit introduces a dUTP-UNG anti pollution system, which can effectively remove residual contamination of PCR products and greatly reduce false positives caused by amplification product contamination.UNG enzyme can rapidly degrade pollutants containing U at room temperature without affecting the formation of new dU based PCR products.

Notes

1. Before using the reagents in this kit, please gently mix them upside down to avoid foaming, and use them after brief centrifugation.
2. This product uses RNA as a template for one-step RT-qPCR experiments. During the operation, RNase contamination should be avoided. It is recommended to perform RNA operations in a specialized area using specialized instruments and consumables. Operators should wear masks and disposable gloves, and frequently change gloves. The experimental consumables should be treated with 0.1% DEPC (diethyl pyrocarbonate) aqueous solution at 37℃ for 12 hours and sterilized under high pressure for 30 minutes before use.
3. The reagents in this kit should avoid repeated freezing and thawing; This product can be stored at -20℃ for long-term storage. If frequent use is required in the short term, it can be stored at 2-8℃.
4. This reagent kit must use specific primers, and the selection of primers can be based on specific experiments. The quality of primer design directly affects the results of RT-qPCR reaction. When designing primers, factors such as GC content, primer length, primer position, and the secondary structure of PCR products need to be considered. It is recommended to use professional primer design software for design.
5. It is recommended to use specific probes in this kit and use professional design software for design