Manual

Product Number: PCK47

Shipping and Storage

-20°C

Components

Description

Super Fi Amplification Mix for NGS is a PCR amplification premix that combines fidelity and amplification specificity, suitable for PCR amplification of high-throughput sequencing libraries.2×Super HiFi PCR Mix is a 2×premixed system composed of High-Fidelity DNA Polymerase, dNTP, PCR stabilizers and enhancers, which has advantages such as strong amplification ability, high fidelity, and strong specificity.When performing library amplification reactions, a wide range, no preference, and high yield of library amplification can be achieved, ensuring the accuracy of sequencing results. During the operation, only primers and templates need to be added, simplifying the experimental steps, improving the experimental throughput and repeatability of the results.2×Mix has added unique protective agents, amplification enhancers, and elongation factors. The unique formula makes the entire reaction system very stable and suitable for amplification of different template libraries.

Note

1. It is recommended to use high-quality purified templates and operate according to the instructions.
2. The number of cycles for library amplification is set based on the input amount. If the number of cycles is too low, it will lead to a low concentration of outbound products, while if it is too high, there will be a preference for amplification.
3. Please perform all operations on ice, 2 × After thawing, please thoroughly mix the Super HiFi PCR Mix and store it at -20 ℃ in a timely manner after use.