T4 DNA Ligase for NGS
2024-10-25
Single Cell WGA Kit
2024-10-28Product Number: BS01
Storage Conditions
-20°C.
Components
| Component | BS01 800 U | BS01 4000 U |
| Bst DNA Polymerase, 8 U/μL | 100 μL | 2×250 μL |
| 10×Bst Reaction Buffer | 1.5 mL | 2×1.5 mL |
| 100mM MgSO4 Solution | 1.5 mL | 2×1.5 mL |
Description
Bst DNA Polymerase (Bst), a Large Fragment, is a purified recombinant enzyme expressed by Escherichia coli. Its gene was derived from Bacillus stearothermophilus and some point mutations were carried out on the basis of the original sequence.The protein has 5'→3' DNA polymerase activity, but no 5'→3' and 3'→5' exonuclease activity, and has strong chain replacement activity. It should be used for DNA isothermal amplification (LAMP), multiple displacement amplification (MDA), whole genome amplification (WGA), library sequencing, etc.
Unit definition
The amount of enzyme required to incorporate 10nmol of deoxynucleotides into an acid insoluble substance at 65°C for 30 min is defined as 1 activity unit (U).Thermal inactivation: inactivation after incubation at 80°C for 20min.
Quality Control
After several column purification, its purity was more than 98% detected by SDSPAGE. No exogenous nuclease activity and no host residual DNA were detected.
