Y-chromosome Human DNA Quantitative Kit
2024-10-25
Bst DNA Polymerase
2024-10-28
Product Number: LG0N
Shipping and Storage
Store at -20℃ and transport on dry ice.
Components
| Component | LG0N 1500U | LG0N 7500U |
| T4 DNA Ligase,15 U/μL | 100μl | 500μl |
| 4×T4 DNA Ligase Buffer | 600μl | 2×1.5ml |
Description
T4 DNA Ligase is isolated and purified from Escherichia coli expressing the T4 DNA Ligase gene after induction and expression. It catalyzes the binding reaction of adjacent DNA strands with 5'phosphate groups and 3'hydroxyl groups via phosphodiester bonds. This enzyme can catalyze the connection of flat or sticky end DNA, repairing single stranded incisions in double stranded DNA, RNA, and DNA/RNA hybridization, but has no activity for single stranded nucleotides.
Application
Mainly used for adapter connection during the construction process of NGS Chinese library.
Unit definition
1U refers to the amount of enzyme required to convert 1nmol [32PPi] into Norit absorbable form within 20 minutes at 37°C in the ATP-PPi exchange reaction, equivalent to approximately 200 viscous terminal junction units.
