T4 Polynucleotide Kinase
2024-10-25
Hotstar DNA Polymerase
2024-10-25Product Number: PC72
Shipping and Storage
-20°C
Components
| Component | PC72 150U | PC72 750U |
| T4 DNA Polymerase (3U/μl) | 50 μl | 250 μl |
| 10×T4 DNA Polymerase Reaction Buffer | 1ml | 4×1 ml |
Description
This product is expressed by Escherichia coli, and the source of the expressed gene is T4 bacteriophage. Due to the simultaneous activity of 5'→3' DNA polymerase and 3'→5' DNA exonuclease, T4 DNA polymerase can be used to flatten the 5' protruding end or flatten the 3' protruding end. It can also be used for labeling DNA probe synthesis through displacement reactions, analyzing the starting point of mRNA transcription through primer elongation, synthesizing the second strand during site-specific mutagenesis, and cloning PCR products that do not rely on linkage reactions.The 3'→5' DNA exonuclease activity of this T4 DNA polymerase is about 100-1000 times higher than Klenow Fragment, and it has higher activity for single stranded DNA than double stranded DNA. This enzyme does not contain exonuclease activity of 5'→3' DNA, and can be inactivated by heating at 70℃ for 10 minutes. Metal ion chelating agents can inhibit its activity.
Unit definition
The amount of enzyme required to incorporate 10 nmol of whole nucleotides into acid insoluble precipitate is defined as 1 active unit (U) within 30 minutes, using thermally denatured calf thymus DNA as a template/primer, at 37℃ and pH 8.8.
Quality control
2U of this enzyme reacted with 1μg of Closed Circular (RFI) pBR322 DNA at 37℃ for 16 hours, and the electrophoresis bands of the DNA remained unchanged.
