Product Number: PCK243

Shipping and Storage

Please send in dry ice and immediately store all components in a -20℃ constant temperature refrigerator after receiving the reagent kit, which can be stored for 6 months. If you need to store it for a longer period of time, please store it below -70℃.

Components

Description

The single-cell whole genome amplification kit is based on an MDA isothermal amplification system, which can achieve whole genome amplification using single cells or trace samples as templates. The amplification product size of a single-cell whole genome after amplification is between 2-100 kb, which can be widely used for second-generation sequencing, large segment copy number variation analysis, microsatellite analysis, qPCR analysis, gene chip analysis, etc.

The Phi29 DNA polymerase used in this kit is a DNA polymerase cloned from bacteriophages, which has strong chain displacement activity and affinity. A single polymerization reaction can achieve continuous polymerization extension of up to 100 kb. Its amplification products are suitable for various downstream applications. Phi29 DNA polymerase also has strong 3 '-5' exonuclease activity, ensuring high fidelity of DNA synthesis. Under normal circumstances, a reaction can produce genomic DNA with a high coverage greater than 20μg.

Self provided instruments and reagents

1. Centrifuge
2. Water bath or PCR instrument
3. Reaction tube: It is recommended to use a low adsorption PCR tube
4. Gun tip: It is recommended to use high-quality filtering gun tips to prevent contamination
5. Deionized water

Note

1. This product has extremely high detection sensitivity, and the experimental operation should be completed in a positive pressure ultra clean workbench. The concentration of amplification reaction products is high, and isolation should be done to avoid aerosol pollution caused by amplification products.
2. Using low-quality samples as templates can affect the quality of the final amplification product, and it is advisable to avoid using a large amount of degraded and fragmented DNA as starting samples.

Operation process diagram