Manual

Product Number: PCK41

Shipping and Storage

-20°C.

Components

Description

This kit uses the method of adding a poly (A) tail at the 3 'end of miRNA to make miRNA have a Poly (A) tail, and then uses Oligo (dT) - Universal tag universal reverse transcription primer for reverse transcription reaction to finally synthesize the first strand cDNA corresponding to miRNA.

The miRNA cDNA first strand synthesis kit contains all the reagents required for the miRNA 3 'end Poly (A) tail modification process and post modification reverse transcription process.This kit has very high Poly (A) modification and reverse transcription efficiency, and can effectively obtain the first strand of cDNA corresponding to miRNA from 1ng-2μg of total RNA.And the operation is simple and fast, which can be used to simultaneously detect multiple miRNAs from a single reaction synthesized cDNA. This not only reduces errors and saves samples, but also achieves high throughput detection.

Note

This kit must be used in conjunction with the miRNA fluorescence quantitative detection kit.

Self prepared experimental material

1ng-2μg of Total RNA , or 0.1ng-1μg of Small molecule RNA.

Note

To prevent RNase pollution, attention should be paid to the following aspects:

1. Use plastic products and gun heads without RNase to avoid cross contamination.
2. Glassware should be dried at a high temperature of 180℃ for 4 hours before use, while plastic utensils can be soaked in 0.5M NaOH for 10 minutes, thoroughly rinsed with water, and then sterilized under high pressure.
3. The solution should be prepared using water without RNase.
4. Operators should wear disposable masks and gloves, and change gloves frequently during the experiment.