Manual

Product Number: PCM19

Shipping and Storage

Store at -20°C.

Components

Description

This product is a kit for quick reverse transcription. 5×Quick RT MasterMix contains all the components needed for first strand cDNA synthesis. The efficiency and yield of cDNA firststrand synthesis are higher, and the first strand of cDNA can be synthesized using pg total RNA or mRNA. The reaction can be completed in 15 minutes. This kit is suitable for the high throughout synthesis of first-strand cDNA and subsequent RT-PCR, RT-qPCR, and construction of full-length cDNA libraries.

Features

1. Ready and easy to use: The reaction will be initiate by adding water and RNA template to the mastermix.
2. Rapid reverse transcription: It takes only 15 minutes to obtain the first strand of cDNA.
3. High sensitivity: pg of total RNA or mRNA can be used as template.
4. High efficiency of reverse transcription: Above 90% RNA will be reverse transcripted.

Note

1. RNase contamination should be avoided during operation to prevent RNA degradation or cross-contamination in experiments. We suggest that the RNA experiments should be performed in a specialized area with specialized equipment and consumables. The operator should wear a mask and disposable gloves and change gloves frequently.
2. The reaction should be set up on ice to prevent RNA degradation. The enzymes should be returned to -20°C as soon as possible after use to avoid repeated freezing and thawing.
3. For RNA templates with complex secondary structures, it is recommended to incubate the template RNA for 5 minutes at 65°C first, then place it on ice immediately, and centrifuge briefly for further processing.