Manual

Product Number: PCK74

Shipping and Storage

-20°C.

Components

Description

This kit is specially developed for one-step RT-PCR experiments. Reverse transcription and PCR are carried out in the same reaction system. There is no need to add reagents or open the tube cover during the reaction process, which improves the detection sensitivity and experimental efficiency while avoiding contamination. This kit includes a new high-efficiency reverse transcriptase, a rapid hot start DNA polymerase, and a reaction buffer for reverse recording and PCR amplification, as well as other components necessary for assays. SuperRT reverse transcriptase RNase H is inactive, which reduces the degradation of RNA in the reverse transcriptase reaction. The reverse enzyme has high reverse transcriptional efficiency and can perform a good reverse transcriptional response to a small amount of RNA template. The rapid hot start DNA polymerase used in PCR reaction has the advantages of high amplification efficiency, strong specificity and fast elongation. The unique buffering system maximizes both reverse transcriptase and polymerase. PCR products can be used directly for T/A cloning because most PCR products obtained by amplification are attached to the "A" base at the 3' end.

Note

1. RNase contamination should be avoided during operation to prevent RNA degradation or cross-contamination in experiments. It is recommended that RNA manipulation be carried out in special areas, using special instruments and consumables, and that operators wear masks and disposable gloves and change gloves frequently.
2. Disposable plastic utensils should be used as far as possible in the experiment. If using glassware, 0.1% DEPC (diethyl pyrocarbonate) aqueous solution should be treated at 37°C for 12 hours and autoclaved at 120°C for 30 minutes before use, or the glassware should be sterilized at 180°C for 60 minutes after dry heat. The sterile water used in the experiment should be autoclaved with 0.1% DEPC.
3. Please mix all reagents in this kit upside down gently before use, avoid foaming as far as possible, and use after a short centrifugation. The enzymes involved should be returned to -20°C as soon as possible after use to avoid repeated freeze-thaw.
4. Specific primers must be used in this kit, and the selection of primers can be selected according to specific experiments. The design of primers directly affects the results of RT-PCR reaction. When designing primers, GC content, primer length, primer location, secondary structure of PCR products and other factors should be considered.