2x E Taq Master mix, with blue dye
2024-10-22
One Step Super RT-PCR Kit
2024-10-22Product Number: PCM12
Shipping and Storage
-20℃.
Components
| Component | PCM12 (1mL) | PCM12 (5mL) | PCM12 (25mL) |
| 2×E Taq Master mix, without dye | 1mL | 5×1mL | 5×5mL |
| ddH2O | 1mL | 5×1mL | 5×5mL |
Note: 2×E Taq Master mix, without dye contains E Taq DNA Polymerase, 3mM MgCl2 and 400μM each dNTP.
Description
This product is a premixed system composed of E Taq DNA Polymerase, Mg2+, dNTPs, PCR stabilizers and enhancers, with a concentration of 2×.E Taq DNA Polymerase has excellent performance of high amplification efficiency and low mismatch rate. The original MasterMix formula makes the entire reaction system very stable, with over 98% of PCR amplification being successful at once. At the same time, complex templates can also be effectively amplified, and human error and pollution can be minimized. This product does not contain dyes. After the PCR program is completed, an appropriate amount of sample loading buffer can be added as needed for electrophoresis operation. Most of the PCR products obtained by amplification have an "A" base attached to the 3 'end, so they can be directly used for T/A cloning. Mainly suitable for experiments such as routine PCR reactions and gene cloning with high fidelity requirements
Quality Control
No exogenous nuclease activity was detected; no host DNA was detected by PCR; single copy genes in multiple genomes could be efficiently amplified.
