One Step RT-qPCR SYBR Kit
2024-10-22
2x E Taq Master mix, with blue dye
2024-10-22Product Number: PC88
Shipping and Storage
-20°C
Components
| Component | PC88 500U | PC88 2500U | PC88 10000U |
| E Taq DNA Polymerase, 5U/μl | 100μl | 5×100μl | 2×1ml |
| 10×PCR Buffer | 1.8 ml | 5×1.8ml | 8×5ml |
Note:The 10×PCR Buffer of this product contains 15mM Mg2+.
Description
E Taq DNA Polymerase is an optimized mixed enzyme of Taq and Pfu DNA Polymerase, with 5'→3' DNA polymerase activity, 5'→3' exonuclease activity, and 3'→5' exonase activity. Compared with Taq DNA Polymerase, E Taq DNA Polymerase has excellent performance of high amplification efficiency and low mismatch rate, and can efficiently amplify DNA fragments. Most of the PCR products amplified with this product have an "A" base at the 3'end, which can be directly used for T/A cloning. This product is suitable for routine PCR reactions and gene cloning with high fidelity requirements.
Unit definition
Using activated salmon sperm DNA as template/primer, the amount of enzyme required to incorporate 10nmol of deoxynucleotides into acid insoluble substances within 30 minutes at 74°C was defined as 1 activity unit (U).
Quality Control
After several column purifications, the purity of the SDS-PAGE test is more than 99%; no exogenous nuclease activity is detected; no host residual DNA is detected by PCR method; it can effectively amplify single-copy genes in the human genome; it can be stored at room temperature for one month , no significant activity changes.
