Manual

Product Number: TR3091

Shipping and Storage

1. This product is transported at room temperature and stored for a long time at 4 ℃, with a validity period of 12 months.
2. This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

EZ virus Transmission Kit is an enhancer developed and synthesized by our biological company for viral infections, which is synthesized using nanotechnology. The EZ virus transmission kit enriches the virus on the cell surface through physical interactions to enhance infection efficiency. Due to the application of nanotechnology, the EZ virus transmission kit not only significantly improves the efficiency of virus infection, but also has very low cytotoxicity and does not interfere with cellular physiological functions.

Note

1. After using the EZ virus Transmission kit for enhancement, the cell density during infection has an impact on the infection effect. It is recommended not to use excessively high cell density for infection.
2. Before using the EZ virus transmission kit enhancer, the optimal MOI (Multiplicity of Infection) of infected cells should be determined, which refers to the average number of viruses infected per cell. Generally, the higher the MOI, the more viruses integrate into chromosomes and the higher the expression level of the target protein. Different cells have their optimal MOI values. We need to conduct a MOI gradient experiment first to determine the optimal MOI value for infected cells. The application of enhancers on the basis of the optimal MOI value has a better effect.
3. The dilution solution for EZ virus transmission kit and virus is a serum-free liquid that is consistent with the cell base culture medium, such as serum-free DMEM or 1640.
4. The relationship between EZ virus transmission kit and infection efficiency. Under other fixed conditions, the larger the amount of enhancer used, the higher the infection efficiency. Generally, the amount of enhancer used can be 1-3 times the recommended amount. However, excessive use of enhancers may lead to cytotoxicity.

Other related reagents

TR3012: Chronic virus infection enhancement reagent.

TR3011: Adenovirus infection enhancing reagent.

TR3003: Adenovirus infection enhancing reagent.

TR3041: Retroviral infection enhancing reagent.

TR3015: Virus infection enhancing reagent in animal bodies.