Manual

Product Number: TR3003

Shipping and Storage

This product is transported at room temperature and stored for a long time at 4℃, with a validity period of 12 months.

This product is safe to use and no biological or chemical toxicity has been found. If accidentally contaminated, rinse with clean water.

Description

EZ-AAV Transmission Kit is an enhancer developed and synthesized by our company for adeno-associated virus infection, which is synthesized using nanotechnology. The EZ-AAV Transmission kit enriches the virus on the cell surface through physical interactions to enhance infection efficiency. Due to the application of nanotechnology, the EZ-AAV Transmission kit significantly improves the efficiency of virus infection while minimizing cell toxicity and not interfering with cellular physiological functions.

Note

1. After using the EZ-AAV Transmission kit for enhancement, the degree of cell fusion during infection has an impact on the infection effect. It is recommended to conduct infection experiments when the degree of cell fusion is around 50%.
2. Before using the EZ-AAV Transmission Kit enhancer, the optimal MOI (Multiplicity of Infection) of infected cells should be determined, which refers to the average number of viruses infected per cell. Generally, the higher the MOI, the more viruses integrate into chromosomes and the higher the surface expression of the target protein. Different cells have their optimal MOI values. We need to conduct a MOI gradient experiment first to determine the optimal MOI value for infected cells. The application of enhancers on the basis of the optimal MOI value has a better effect.
3. The dilution solution for EZ-AAV Transmission kit and virus is a serum-free liquid that is consistent with the cell base culture medium, such as serum-free DMEM or 1640.
4. The relationship between EZ-AAV Transmission Kit and infection efficiency. Under other fixed conditions, the larger the dosage of enhancers, the higher the infection efficiency. Generally, the dosage of enhancers can be used 1-3 times the recommended dosage. However, excessive use of enhancers may lead to cytotoxicity.
5. After using the EZ-AAV Transmission kit, sodium butyrate can also be used simultaneously.

Other related reagents

TR3011: Adenovirus infection enhancing reagent.

TR3012: Chronic virus infection enhancement reagent.

TR3041: Retroviral infection enhancing reagent.

TR3015: Virus infection enhancing reagent in animal bodies.