Manual

Product Number: ATA087

Shipping and Storage

-20°C.

Components

Description

Taq Antibody is a mouse monoclonal Antibody against Taq DNA Polymerase and applies to Hot Start PCR. When combined with the Taq DNA Polymerase, Taq Antibody inhibits DNA polymerase activity, which in turn inhibits non-specific annealing of primers and non-specific amplification induced by primer dimers at low temperatures. Taq Antibody denatures during the initial DNA denaturation step of the PCR reaction, when DNA polymerase activity is restored to achieve a heat-start effect. Therefore, there is no need for special inactivation of Taq antibody.

Features

1. At 37°C, >95% polymerase activity can be inhibited.
2. It can improve the specificity and sensitivity of PCR reaction, including complex human genomic DNA or cDNA template, low copy number DNA or RNA, multiplex PCR, etc.
3. PCR reaction rate is faster than common chemically modified polymerase.

Unit definition

After incubation at 25°C for 15 min, 1U Taq Antibody is defined to inhibit more than 97% of the 1U Taq DNA Polymerase activity at 37°C for 30 min.