Manual

Description

Taq DNA Polymerase Isolated from the E. coli cloning Thermus aquaticus. The molecular weight is approximately 94 kDa. EasyTaq DNA polymerase has the 5' to 3' DNA polymerase activity and 5' to 3' exonuclease activity without 3' -5' exonuclease activity. The extending speed is 1-2 kb/min. There is an "A" on 3' end. The PCR product can be cloned in TA vector.

Conc. 5 U/μl

Store at -20°C

Characteristics

1.high-sensitivity
2.high amplification efficiency

Unit Definition

One unit of Platinum Taq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Quality Control

• functional absence of double- and single-stranded endonuclease activity;
• Purity>90% homogeneous by SDS gel electrophoresis.
• Each lot of EasyTaq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA.

Storage Buffer

20 mM Tris-HCl (pH 8.0),0.1 mM EDTA,1 mM DTT,100 mM KCl,Stabilizers,50% glycerol.

10X PCR reaction Buffer

500 mM KCl,100 mM Tris-Cl (pH8.5 at 25°C), 1% Triton X-100,15 mM MgCl2

Reaction Mixture Set Up

Component	Volume	Final Concentration
Template	DNA <0.5 ug	as required
Forward Primer (10 μM)	1 μl	0.2-0.4 μM each
Reverse Primer (10 μM)	1 μl	0.2-0.4 μM each
10xPCR reaction Buffer	5 μl	1x
2.5 mM dNTPs	4 μl	0.2 mM
Taq DNA polymerase	0.5μl	2.5 unit
ddH2O to final volume	50μl	Not applicable

Recommended thermal cycling conditions

94°C 2-5 min
94°C 30 sec
50-60°C 30 sec 30-35 cycles
72°C 1-2 kb/min
72°C 5-10 min

Package Details

Bulk package Store at -20 degree With low temperature shipping

Order

PC01	Taq DNA Polymerase	Taq DNA Polymerase 5U/μl standard package 1000U/vial or bulk, supplied with PCR Bufer
PC02	Taq DNA Polymerase(10U/μl)	Taq DNA Polymerase 10U/μl standard package 5000U/vial or bulk