Manual

Description

2x Ultra SYBR Mixture (With Low ROX) is a convenient premixed 2x concentrated solution for SYBR Green I-based qPCR which include HotStar Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg²⁺ and ROX calibration dye. It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to double-stranded (ds) DNA without using sequence-specific probes. The chemically modified HotStar Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes. The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity.

The ROX is a dye molecule that can be used to normalize the well-to well fluorescent fluctuations. It is the low concentration in this kit. This kit applied to ABI Prism 7500/7500 Fast, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000 and other low rox level PCR instructions.

Packing

1ml or Bulk

Application

Gene expression analysis
Gene copy number analysis

Features

High sensitivity: exactly quantitate the low copy template
High specificity: maximatily reduce the primer dimers and non-specific product
Wide linear range; Accurate quantitation.

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