2x E Taq PCR Master Mix Lyophilized Powder

E Taq Master Mix Lyophilized Powder

Description

E Taq Master Mix (Lyophilized Powder) uses advanced lyophilization technology made to lyophilized powder format. The lyophilized E Master mix and the 2x E Master buffer for LP when keep at room temperature is very stable, without any activity lost. The 2x E Taq Master Mix contain E DNA Polymerase, Mg²⁺, dNTPs and special stabilizer and PCR enhancer, concentration is 2x E Taq DNA Polymerase has the Excellent performance of high Amplification Efficiency and low mismatch rate. The original Master Mix formula makes the reaction system very stable, more than 98% PCR amplification can be successful at one time. At the same time, the complex template can be amplified effectively and minimize human error and pollution. This product does not contain dyes. After the completion of the PCR procedure, a suitable amount of sample loading buffer is needed according to the need for electrophoresis operation. Most of the amplified products have a "A" base at the 3' end, so they can be directly used in T/A cloning.

Packing size

1ml or 5ml

Components

1vial E Taq Master Mix LP with 1vial of dilution buffer

Quality Control

No exogenous nuclease activity, no host DNA detected by PCR, various genomic single copy DNA can be amplified effectively.

Protocol

Pre preparation Add 2x E Taq Master buffer for LP to E Taq Master LP, Vortex oscillation until complete dissolution.
After it completely dissolved, transferre the solution from cillin bottle to a 2x Es Taq Master buffer for LP tube, then make a mark on it.
The mixed solution needs to be stored at -20°C for a long time storage, if frequency use, it can be stored at 2-8°C.
The following example is applied to conventional PCR reaction system and reaction conditions and it can be modified and optimized according to the template, primer structure and target fragment size.
Template DNA Human genomic DNA, size 1KB

Shipping And Storage

E Taq Master LP and 2x E Taq Master buffer for LP can store at room temperature.
When E Taq Master LP is mixed with 2 x E Taq Master buffer for LP, it needs to be stored at -20°C
If frequently use, Store at 2-8°C for short-term (1 week), and avoid freeze thawing

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